|
KMID : 0525720100150040195
|
|
Journal of Chitin and Chitosan
2010 Volume.15 No. 4 p.195 ~ p.202
|
|
|
Significance of the Molecular Weight and Degree of N-Deacetylation of Chitosan-Oligosaccharides on the Biological Applications
|
|
Park Jae-Kweon
Jo Hang-Soo
Lee Choul-Gyun
Park Yong-Il
|
|
|
Abstract
|
|
|
In order to investigate the potential of chitosan-oligosaccharides (CTSN-OS) prepared by enzymatic digestion of high
molecular-weight chitosan, the antiviral effect of CTSN-OS was studied. Their molecular weights and degree of N-deacetylation were primarily determined by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The CTSN-OS were then further fractionated into two groups by gel-filtration: 1) oligosaccharides with no N-acetyl groups, consisting of (GlcN)n, n=3-5 (trimer; 31.7%, tetramer; 37.7%, and pentamer; 18.6%, hereafter referred to as COS, and 2) those with more N-acetylated residues, consisting of a mixture of (GlcN)n, n>5 and (GlcNAc)n, n=1-3 in various ratios, hereafter referred to as HOS. The HOS showed higher molecular weights than COS. The inhibitory effects of CTSN-OS, COS and HOS on neuraminidase and antiviral activity against herpes simplex virus type-1 (HSV-1) were investigated in vitro. The results demonstrated that HOS showed lower inhibitory activities toward both neuraminidase and HSV-1 than inhibitory activities of CTSN-OS that showed toward neuraminidase and HSV-1 whereas COS showed similar effects on both neuraminidase and HSV-1 to those of CTSN-OS, respectively. CTSN-OS or COS has not shown the significant inhibitory activities though, these results suggest that lower molecular weights and/or a lower degree of N-acetylation of chitosan-oligosaccharides are important factors giving rise to their antiviral activities. In addition, in order to elucidate the biological function and utilization of CTSN-OS, transformation of the green algae Haematococcus lacustris with a phytoene desaturase gene involved in astaxanthin biosynthesis prior to establish the condition for si-RNA transformation to elucidate the pathway was performed using CTSN-OS and/or high molecular weight chitosan as a gene carrier. As the results of comparison, CTSN-OS that has shown the higher DNA binding capacity than the capacity with high molecular weight chitosan did affect on cell growth and the production of extracellular materials instead showing the efficiency of the transformation in the present study.
|
|
|
KEYWORD
|
|
|
Chitosan-oligosaccharide (CTSN-OS), deacetylation degree, molecular weight, antiviral activity, gene carrier
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|